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Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
JoVE 杂志
化学
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JoVE 杂志 化学
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

13,768 Views

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10:23 min

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August 26, 2013

DOI:

10.3791/50842-v

DOI:
10.3791/50842-v

•

10:23 min
August 26, 2013

•

1 Views
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1Department of Biophysical Chemistry, Institute for Physical Chemistry,University of Heidelberg, 2Max Planck Institute for Intelligent Systems at Stuttgart

Summary

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We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.

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Cite this Article

Pohl, T. L. M., Schwab, E. H., Cavalcanti-Adam, E. A. Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach. J. Vis. Exp. (78), e50842, doi:10.3791/50842 (2013).

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