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Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
JoVE 杂志
生物学
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JoVE 杂志 生物学
Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

DOI:
10.3791/50840-v

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12:38 min

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December 18, 2013

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, , , , ,
1Department of Life Sciences, National Institute for Biotechnology in the Negev,Ben-Gurion University of the Negev

Chapters

  • 00:05Title
  • 01:20Monitoring Behavior by Thermo-resistance
  • 02:35Monitoring Protein Localization by Immunostaining
  • 07:39Monitoring Protein Folding and Stability by Partial Digestion
  • 09:46Monitoring Protein Folding and Stability in a Multicellular Organism
  • 12:11Conclucion

Summary

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To study the relationship between protein homeostasis, stress and aging, we monitored changes in protein folding by following protein dysfunction, protein localization in the cell and protein stability at the organismal, cellular and protein levels, using the genetically tractable metazoan Caenorhabditis elegans as a model system.

Tags

Keywords: Protein HomeostasisProteostasisCaenorhabditis ElegansProtein FoldingProtein FunctionProtein MisfoldingMetastable ProteinsBehavioral AssaysImagingBiochemical ApproachesTemperature-sensingDevelopmentAgingMetabolism

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