需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Chapters
- 00:05Title
- 01:27Depletion and Isolation of PBMCs via Ficoll Separation
- 02:08Antibody Blockade and Antigen-Specific Stimulation of Cells
- 02:46Collection and Analysis of Supernatant
- 03:15Collection and Phenotypic Analysis of Cells via Flow Cytometry
- 04:09Analysis of Cells via qRT-PCR
- 05:15Adaptation for Intracellular Cytokine Staining at 48 Hours
- 05:48Adaptation for Sorting Cells
- 06:55Results: HIV-specific CD4 T Cell Effector Function
- 09:03Conclusion
