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Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
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Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

DOI:
10.3791/50794-v

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09:42 min

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December 08, 2013

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,
1Center for Research on Occupational and Environmental Toxicology,Oregon Health & Science University, 2Department of Behavioral Neuroscience,Oregon Health & Science University

Chapters

  • 00:05Title
  • 01:49Preparation of Hypothalamic Brain Slices
  • 04:29Patch-clamp Recording
  • 06:32Measurement of Intracellular Ca2+
  • 07:38Results: Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
  • 09:05Conclusion

Summary

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Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.

Tags

Keywords: Simultaneous ElectrophysiologyCalcium ImagingSuprachiasmatic NucleusCircadian ClockSynaptic TransmissionCalcium SignalingPatch ClampBrain Slice

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