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Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

19,295 Views

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11:53 min

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November 04, 2013

DOI:

10.3791/50631-v

DOI:
10.3791/50631-v

•

11:53 min
November 04, 2013

•

5 Views
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1Department of Biology,University of Kentucky, 2Department of Biology, College of Science,University of Salahaddin, Iraq, 3Department of Neurobiology and Cognitive Neuroscience,SISSA, Italy

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This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.

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Intracellular RecordingSensory Field MappingNeuron CulturingHirudo MedicinalisLeechNeurophysiologyNeuroethology发育生物学Nervous SystemElectrophysiologyGanglionNeural CircuitsCell CultureSensory FieldMotor FieldNeural Networks行为学SynaptogenesisDevelopmentTeaching Laboratory

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Titlow, J., Majeed, Z. R., Nicholls, J. G., Cooper, R. L. Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis. J. Vis. Exp. (81), e50631, doi:10.3791/50631 (2013).

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