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Motor Nerve transection og Time-lapse Imaging af gliacelle Behaviors Live zebrafisk
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神经科学
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JoVE 杂志 神经科学
Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish

Motor Nerve transection og Time-lapse Imaging af gliacelle Behaviors Live zebrafisk

DOI:
10.3791/50621-v

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09:05 min

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June 20, 2013

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,
1Department of Biology,University of Virginia

Chapters

  • 00:05Title
  • 01:18Preparation and Mounting of Zebrafish Embryos
  • 03:10Laser Calibration and Testing
  • 04:35Nerve Transection Using Laser Ablation and Time-lapse Confocal Imaging of Glial Cell Behaviors
  • 06:39Representative Laser Ablation Results
  • 08:39Conclusion

Summary

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Selvom det perifere nervesystem (PNS), er i stand til væsentlig reparation efter skade, er lidt om de cellulære og molekylære mekanismer, der styrer dette fænomen. Ved hjælp af levende, transgene zebrafisk og en reproducerbar nerve transection assay, kan vi studere dynamiske glialcellelinjer adfærd under nerve degeneration og regeneration.

Tags

Keywords: Motor Nerve TransectionTime-lapse ImagingGlial Cell BehaviorsZebrafishPeripheral Nervous SystemRegenerationAxonSchwann CellsPerineuriumAxotomyIn Vivo ImagingGenetic Manipulation

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