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Implementazione morsetto dinamico con conduttanze sinaptiche e artificiale nel topo cellule gangliari della retina
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神经科学
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JoVE 杂志 神经科学
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
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Implementazione morsetto dinamico con conduttanze sinaptiche e artificiale nel topo cellule gangliari della retina

12,157 Views

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11:46 min

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May 16, 2013

DOI:

10.3791/50400-v

DOI:
10.3791/50400-v

•

11:46 min
May 16, 2013

•

2 Views
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1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney , 2The MARCS Institute,University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney

Summary

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In questo articolo il video illustra il set-up, le procedure per applicare le patch corpi cellulari e come implementare le registrazioni dinamiche clamp da cellule gangliari in tutto il montaggio del mouse retine. Questa tecnica permette l'indagine del contributo preciso di input sinaptici eccitatori e inibitori, e la loro portata, il tempo di spiking neuronale.

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Cite this Article

Huang, J. Y., Stiefel, K. M., Protti, D. A. Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells. J. Vis. Exp. (75), e50400, doi:10.3791/50400 (2013).

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