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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
JoVE 杂志
生物工程
Author Produced
This content is Free Access.
JoVE 杂志 生物工程
Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

DOI:
10.3791/50360-v

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15:14 min

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April 11, 2013

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, ,
1Entomology,International Center for Tropical Agriculture (CIAT), Cali, Colombia , 2Sustainable Perennial Crops Laboratory, Agricultural Research Service,United States Department of Agriculture, Beltsville, Maryland, USA

Chapters

  • 00:02Title
  • 00:04Introduction
  • 02:30Plants
  • 03:51Fungus
  • 08:22Inoculations
  • 09:31Evaluations
  • 12:43Results
  • 13:46Conclusions

Summary

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This protocol demonstrates two inoculation methods to introduce the fungal entomopathogen Beauveria bassiana as an endophyte in the common bean (Phaseolus vulgaris), in preparation for subsequent evaluations of endophytic biological control.

Tags

Keywords: Fungal EntomopathogenBeauveria BassianaEndophytic ColonizationBiological ControlInoculation MethodsSurface SterilizationFungal Growth Assessment

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