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High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

34,120 Views

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10:47 min

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June 11, 2013

DOI:

10.3791/50356-v

DOI:
10.3791/50356-v

•

10:47 min
June 11, 2013

•

10 Views
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1Application Development,Akonni Biosystems, Inc., 2Manufacturing,Akonni Biosystems, Inc., 3工程学,Akonni Biosystems, Inc., 4Research & Development,Akonni Biosystems, Inc.

Summary

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TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.

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TruTip TechnologyNucleic Acid ExtractionAutomated Sample ProcessingClinical SamplesLiquid Handling RobotsRNA IsolationGenomic DNA IsolationFetal DNA ExtractionEppendorf EpMotion 5070Hamilton STARSTARplusHigh-throughputPorous Monolithic Binding MatrixCell LysisNucleic Acid BindingWashingElution

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Holmberg, R. C., Gindlesperger, A., Stokes, T., Brady, D., Thakore, N., Belgrader, P., Cooney, C. G., Chandler, D. P. High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots. J. Vis. Exp. (76), e50356, doi:10.3791/50356 (2013).

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