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Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior
JoVE 杂志
行为学
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JoVE 杂志 行为学
Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

DOI:
10.3791/50336-v

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12:20 min

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June 10, 2013

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, , , , , , ,
1Department of Oral and Maxillofacial Surgery,University of Florida College of Dentistry, 2Department of Neuroscience, McKnight Brain Institute,University of Florida College of Medicine , 3Stoelting Co., 4Department of Orthodontics,University of Florida

Chapters

  • 00:05Title
  • 01:20Training and Baseline Sessions
  • 01:54Programming the OPAD System for Protocols and Experiments
  • 05:49Pretest Preparation and Capsaicin Treatment
  • 07:02Running the Assay
  • 09:40Data Analysis with OPAD Software
  • 10:40Rodent Behavior on the OPAD
  • 11:57Conclusion

Summary

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We present a user-friendly, high-throughput operant system for the evaluation of pain behaviors in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) can assess pain through a reward/conflict paradigm thus providing a more humane way of testing. This protocol will yield more clinically relevant and translational data from rodents.

Tags

Operant Orofacial Pain Assessment Device (OPAD)Nociceptive BehaviorPain AssessmentRodentsPeltier-based ThermodesReward BottleLicksFace ContactsLick/face RatioHigh-throughputOperant AssayCortical InputSpinal Reflex-based Nociceptive Assays

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