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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

DOI:
10.3791/50242-v

•

07:34 min

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March 14, 2013

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, ,
1Proteomics and Metabolomics Facility,Colorado State University

Chapters

  • 00:05Title
  • 00:56Sample Organization
  • 02:09Serum and Quality Control (QC) Sample Preparation Procedures
  • 03:25UPLC-MS Data Acquisition
  • 04:53Metabolite Identification
  • 06:03Results: Identification of a Caffeine Metabolite in Human Serum
  • 06:51Conclusion

Summary

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Non-targeted metabolite profiling by ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS) is a powerful technique to investigate metabolism. This article outlines a typical workflow utilized for non-targeted metabolite profiling of serum including sample organization and preparation, data acquisition, data analysis, quality control, and metabolite identification.

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Metabolomic ProfilingNon-targeted AnalysisUPLC-MSSerumAnalytical WorkflowSample PreparationData AcquisitionQuality ControlMetabolite Identification

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