需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster
Chapters
- 00:05Title
- 01:22Preparation of 10 µM All-Trans-Retinal Food
- 01:49Generation of Channelrhodopsin Flies
- 02:39Experimental Set-up
- 04:46Behavioral Assay
- 05:51Results: Escape Times in Flies Expressing ChR2 in Foma-1 Neurons
- 07:36Conclusion
Genetically encoded optogenetic tools enable noninvasive manipulation of specific neurons in the Drosophila brain. Such tools can identify neurons whose activation is sufficient to elicit or suppress particular behaviors. Here we present a method for activating Channelrhodopsin2 that is expressed in targeted neurons in freely walking flies.
