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Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
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Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

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08:14 min

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February 14, 2013

DOI:

10.3791/50084-v

DOI:
10.3791/50084-v

•

08:14 min
February 14, 2013

•

2 Views
,
1Plant Sciences Institute,Agricultural Research Service, United States Department of Agriculture, 2Molecular Plant Pathology Laboratory,Agricultural Research Service, United States Department of Agriculture

Summary

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A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.

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Virus-like ParticlesMaize Rayado Fino VirusSolvent AccessibilityCysteine ResiduesPeptide BioconjugationVirus ModificationVirus-based TechnologiesPlant-produced VLPsVirus Structure And Properties

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Natilla, A., Hammond, R. W. Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs. J. Vis. Exp. (72), e50084, doi:10.3791/50084 (2013).

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