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Real-time Live Imaging of T-cell Signaling Complex Formation
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JoVE 杂志 免疫与感染
Real-time Live Imaging of T-cell Signaling Complex Formation

Real-time Live Imaging of T-cell Signaling Complex Formation

DOI:
10.3791/50076-v

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10:31 min

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June 23, 2013

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, ,
1The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Chapters

  • 00:05Title
  • 01:28T-cell Spreading Assay Preparation
  • 02:52T-cell Activation and Imaging
  • 07:21Imaging Data Analysis
  • 08:42Results: Live Imaging of SLP76 Deficient T-cells
  • 09:48Conclusion

Summary

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We describe a live-cell imaging method that provides insight into protein dynamics during the T-cell activation process. We demonstrate the combined usage of the T-cell spreading assay, confocal microscopy and imaging analysis to yield quantitative results to follow signaling complex formation throughout T-cell activation.

Tags

T-cell SignalingImmune SystemT LymphocytesT-cell ActivationT-cell Antigen Receptor (TCR)Antigen Presenting Cells (APCs)Immune SynapseProtein-protein InteractionsLive Cell ImagingProtein Complex FormationReal-time ImagingJurkat CellsFluorescent Proteins

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