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Chapters
- 00:00Title
- 02:12Methods-Biocontainment Facility
- 02:47Methods-Animal Preparation
- 03:28Methods-Intranasal Infection
- 03:49Methods-Luciferin Injection
- 04:10Preparation for Imaging
- 05:09LivingImage Software Imaging
- 05:39Image Reconstruction
- 06:083-Dimensional Reconstruction
- 09:29Recovery and Cleanup
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over multiple time points ensures reduced animal requirements, costs, and overall morbidity to the animals utilized ensuring a more humane and more scientific means of disease study.
Tags
In Vivo Imaging Systems (IVIS)Neuro-invasive Encephalitic VirusImaging TechnologyViral ResearchAnimal ModelsScientific AccuracyReduction Of Animal UsageStatistical AccuracyVaccine Efficacy StudiesMorbidity And Mortality EndpointsIn Vivo Imaging Systems (IVIS)Firefly Bioluminescent EnzymeCentral Nervous System (CNS)Encephalitic VirusMurine ModelIntranasal InfectionTC83-LucAttenuated Venezuelan Equine Encephalitis VirusLuciferase Gene
