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The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms
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JoVE 杂志 免疫与感染
The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms

The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms

DOI:
10.3791/4370-v

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05:57 min

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July 02, 2013

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, , , , ,
1Department of Physics,Bar-Ilan University, 2The Mina & Everard Goodman Faculty of Life Sciences,Bar-Ilan University, 3Department of Chemistry,Bar-Ilan University, 4Faculty of Engineering,Bar-Ilan University

Chapters

  • 00:05Title
  • 01:03Bacterial Culture
  • 01:47Illumination
  • 02:07Determination of Colony-forming Units (CFU)
  • 03:17Results: The Synergistic Effort of Visible Light and Gentamycin on Pseudomona aeruginosa
  • 05:21Conclusion

Summary

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We show that a developed biomedical device involving continuous or pulsed visible laser based treatment that is combined with antibiotic treatment (gentamycin), results in a statistically significant synergistic effect leading to a reduction in the viability of P. aeruginosa PAO1, by 8 log's compared to antibiotic treatment alone.

Tags

Synergistic EffectVisible LightGentamycinPseudomonas AeruginosaBactericidal EffectBlue LightSpectrumPhototoxic EffectReactive Oxygen Species (ROS)Endogenous Bacterial PhotosensitizersBiocidal EffectRed LightNear Infra Red LightGreen LightHigh Power Green LightContinuous Wave (CW) LightPulsed Q-switched (Q-S) LightAntibiotic TreatmentGentamycinBacteria ViabilityNosocomial Opportunistic PathogenAcrB/Mex-type RND Multidrug Efflux SystemsGram-negative Bacteria

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