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Flowcytometrische Isolatie van primaire Murine Type II alveolaire epitheelcellen voor Functionele en moleculaire studies
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Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
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Flowcytometrische Isolatie van primaire Murine Type II alveolaire epitheelcellen voor Functionele en moleculaire studies

25,868 Views

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14:48 min

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December 26, 2012

DOI:

10.3791/4322-v

DOI:
10.3791/4322-v

•

14:48 min
December 26, 2012

•

23 Views
, , , , ,
1Research Group Immune Regulation,Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology,Otto-von-Guericke University , 3Department of Experimental Immunology,Helmholtz Centre for Infection Research

Summary

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We beschrijven de snelle isolatie van primaire murine type II alveolaire epitheelcellen (AECII) door flowcytometrische negatieve selectie. Deze AECII vertonen hoge levensvatbaarheid en zuiverheid en zijn geschikt voor diverse functionele en moleculaire studies over hun rol bij luchtwegaandoeningen zoals auto of infectieziekten.

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Gereke, M., Autengruber, A., Gröbe, L., Jeron, A., Bruder, D., Stegemann-Koniszewski, S. Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies. J. Vis. Exp. (70), e4322, doi:10.3791/4322 (2012).

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