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免疫与感染

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主要鼠Ⅱ型肺泡上皮细胞的功能及分子生物学研究的流式细胞仪分离

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Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

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主要鼠Ⅱ型肺泡上皮细胞的功能及分子生物学研究的流式细胞仪分离

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14:48 min

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December 26, 2012

DOI:

DOI:

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14:48 min

December 26, 2012

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23 Views

Marcus Gereke^1,2, Andrea Autengruber^1,2, Lothar Gröbe³, Andreas Jeron², Dunja Bruder^1,2, Sabine Stegemann-Koniszewski¹

¹Research Group Immune Regulation,Helmholtz Centre for Infection Research, ²Research Group Infection Immunology, Institute of Medical Microbiology,Otto-von-Guericke University , ³Department of Experimental Immunology,Helmholtz Centre for Infection Research

Summary

我们描述了迅速隔离的主要鼠Ⅱ型肺泡上皮的细胞（AECII）流式细胞仪的负选择。这些AECII显示活力和纯度高，适用于范围广泛的功能和分子生物学研究，他们的作用，自身免疫性疾病或感染性疾病，如呼吸系统疾病。

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