Journal
/
神经科学
/
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
Please note that all translations are automatically generated. Click here for the English version.

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

DOI:
10.3791/4281-v

•

12:43 min

•

October 17, 2012

•

, , , , , , ,
1Natural Science Division,Pepperdine University

Chapters

  • 00:05Title
  • 01:52Pre-experimental Preparations
  • 02:47Isolation of Auditory and Vestibular Labyrinth
  • 05:28Hair Cell Isolation
  • 07:18Patch Clamping Zebrafish Hair Cells
  • 10:12Results: Isolation of and Electrophysiological Recordings from Zebrafish Hair Cells
  • 12:04Conclusion

Summary

自动翻译

自动翻译

The purpose of this video is to demonstrate procedures for obtaining healthy, intact hair cells from the inner ear organs of adult zebrafish and then using them for patch clamp studies aimed at characterizing the biophysical properties of their voltage-gated channels.

Tags

Patch Clamp RecordingsInner Ear Hair CellsZebrafishVoltage-gated ChannelsSensory Hair CellsIsolation MethodHair Cell SizeHair Cell MorphologyDiversityPatch Clamp RecordingsZebrafish Model SystemMutantsHearing And BalanceTransgenic LinesGenetic AnalysisElectrophysiological MethodsSensory Modalities

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/4281?article_type=v

Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation
check_url/cn/4281?article_type=v

Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
check_url/cn/4281?article_type=v

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse
check_url/cn/4281?article_type=v

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
check_url/cn/4281?article_type=v

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
check_url/cn/4281?article_type=v

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
check_url/cn/4281?article_type=v

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
check_url/cn/4281?article_type=v

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
check_url/cn/4281?article_type=v

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
check_url/cn/4281?article_type=v

Whole-cell Patch-clamp Recordings in Brain Slices

Read Article