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电压敏感染料记录单个神经元在大脑切片的轴突,树突和树突棘
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
DOI:

12:51 min

November 29, 2012

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Chapters

  • 00:05Title
  • 02:09Equipment Setup
  • 04:35Whole-cell Patch-clamp Recording and Dye Diffusion
  • 07:35Optical Recording
  • 09:30Results: Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
  • 11:54Conclusion

Summary

自动翻译

一种成像技术用于监测具有亚微米的空间和亚毫秒时间分辨率的膜电位的变化进行说明。技术,基于电压敏感染料激光激发,使测量信号的轴突和轴突抵押品,终端树突分支,和个人的树突棘。

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