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Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans
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生物学
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JoVE 杂志 生物学
Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

DOI:
10.3791/4088-v

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11:07 min

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July 20, 2012

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, ,
1Department of Biochemistry,University of Washington, 2Molecular and Cellular Biology Program,University of Washington

Chapters

  • 00:05Title
  • 01:27Construction of Environmental Chambers
  • 02:56Connecting the Gas Supply to the Environmental Chamber
  • 04:39Preparation of Samples for Viability Assay
  • 07:32Rapid Harvest of Hypoxia-exposed Worms
  • 10:11Results: The Effect of Hypoxia on C. elegans
  • 10:44Conclusion

Summary

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This paper details how to use continuous-flow hypoxia chambers to generate atmospheres with defined concentrations of O2 to understand biological responses to decreased O2. This system is easy to setup and maintain, and flexible enough to suit a wide range of O2 concentrations and model systems

Tags

Defined Gaseous EnvironmentsHypoxiaC. ElegansCellular Signaling PathwaysTreatment StrategiesModel OrganismGenetically AmenableHypoxic ResponseO2 ConcentrationsTranscriptional ResponseHif-1Ppm O2

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