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Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
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JoVE 杂志 生物学
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

DOI:
10.3791/3992-v

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14:38 min

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April 20, 2012

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, ,
1Department of Microbiology,Miami University

Chapters

  • 00:05Title
  • 02:03Sample Acquisition
  • 04:24Development of Enrichment Cultures
  • 06:42Cell Lysate Extraction from Filtered Enrichments
  • 08:44RubisCO Carboxylase Activity Filter Assay
  • 11:17Results: Analysis of Novel Protists from Lake Bonney, Antarctica
  • 13:55Conclusion

Summary

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Microbial eukaryotes are both a source of photosynthetically-derived carbon and top predatory species in permanently ice-covered Antarctic lakes. This report describes an enrichment culture approach to isolate metabolically versatile microbial eukaryotes from the Antarctic lake, Lake Bonney, and assesses inorganic carbon fixation potential using a radioisotope assay for Ribulose-1,5-bisphophate carboxylase oxygenase (RubisCO) activity.

Tags

Microbial EukaryotesAntarctic LakeChemically StratifiedCarbon Fixation PotentialLake BonneyIce-covered LakesMcMurdo Dry ValleysEnvironmental StressesNutrient DeficiencyLow TemperaturesExtreme ShadeHypersalinityDarknessMicroorganismsProtistsBiogeochemical CyclingCarbon CyclingAutotrophyPrimary ProducersTertiary RolesFood WebPhagotrophic ProtistsHeterotrophic ProtistsMixotrophic Metabolism

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