Journal
/
神经科学
/
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
JoVE 杂志
神经科学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

DOI:
10.3791/3907-v

•

09:51 min

•

July 15, 2012

•

, ,
1Fishberg Department of Neuroscience and Friedman Brain Institute,Mt. Sinai School of Medicine, 2Phase Five Communications Inc.

Chapters

  • 00:05Title
  • 01:53Dissection
  • 03:21Preparing electrodes
  • 04:40Loading the calcium indicator dye
  • 05:27Calcium imaging and electrophysiological recording
  • 07:57Analysis
  • 08:40Representative data
  • 09:18Conclusions

Summary

自动翻译

自动翻译

We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.

Tags

Intracellular CalciumSynaptic EfficacyMollusc AplysiaCalcium ImagingIntracellular RecordingBuccal GanglionSynaptic PlasticityHomosynaptic FacilitationCalcium OrangeIontophoresisFluorescent Dye

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Read Article