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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
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March 02, 2012
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Isotachophoresis (ITP) is a robust electrokinetic separation and preconcentration technique with applications ranging from toxin detection to sample preparation. We review the physical principles of ITP and the methodology of applying this technique to two specific example applications: separation and detection of small molecules and purification of nucleic acids from cell culture lysate.
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On-chip IsotachophoresisSeparation Of IonsPurification Of Nucleic AcidsElectrokinetic TechniquesMicroscale ApplicationsFluidic ProcessesElectrophoretic ProcessesIsotachophoresis (ITP)PreconcentrationIonic MobilityUnlabeled Ionic MoleculesSample PreparationExtraction And Purification Of Nucleic AcidsComplex MatricesCell CultureUrineBloodElectric FieldBuffersAnionic Analytes
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Garcia-Schwarz, G., Rogacs, A., Bahga, S. S., Santiago, J. G. On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids. J. Vis. Exp. (61), e3890, doi:10.3791/3890 (2012).
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