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多光子显微镜的清除小鼠脑组织表达YFP
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神经科学
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JoVE 杂志 神经科学
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
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多光子显微镜的清除小鼠脑组织表达YFP

13,681 Views

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10:03 min

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September 23, 2012

DOI:

10.3791/3848-v

DOI:
10.3791/3848-v

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10:03 min
September 23, 2012

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4 Views
, , ,
1Department of Biomedical Engineering,Yale University, 2Department of Biomedical Engineering,Louisiana Tech University

Summary

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整个小鼠脏器的多光子显微镜能够通过光学成像之前清零器官，但并非所有协议保留的荧光蛋白的荧光信号。使用光学乙醇脱水和苯甲醇苯甲酸苄酯结算的结算方法，显示高分辨率的多光子图像的整个小鼠脑组织表达YFP。

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Cite this Article

Parra, S. G., Vesuna, S. S., Murray, T. A., Levene, M. J. Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP. J. Vis. Exp. (67), e3848, doi:10.3791/3848 (2012).

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