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Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
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11:45 min
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March 08, 2012
DOI:
1Department of Pathology,New York University Langone School of Medicine, 2Program in Molecular Pathogenesis,Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, 3Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases,National Institutes of Health, 4Veteran Affairs New York Harbor Healthcare System
Summary
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This article describes a method to visualize formation of an HIV-1 envelope-induced virological synapse on glass supported planar bilayers by total internal reflection fluorescence (TIRF) microscopy. The method can also be combined with immunofluorescence staining to detect activation and redistribution of signaling molecules that occur during HIV-1 envelope-induced virological synapse formation.
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HIV-1EnvelopeVirological SynapseSignalingSynthetic Lipid BilayersCell To Cell TransmissionCD4+ T CellsF-actinGp120Chemokine Receptor (CKR)CCR5CXCR4LFA-1ICAM FamilyVirus-infected Donor CellsTarget CellsHIV-1 VirionsIntracellular Signaling EventsTCR SignalosomeLckCD3ζZAP70LATSLP-76ItkPLCγSupramolecular Arrangement
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Prins, K. C., Vasiliver-Shamis, G., Cammer, M., Depoil, D., Dustin, M. L., Hioe, C. E. Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers. J. Vis. Exp. (61), e3757, doi:10.3791/3757 (2012).
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