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Assay Neuroblast: Assay לדור והעשרה של ובתאים העצבית הבחנה בין צאצאיהם גזע עצביים Cell שימוש cytometry זרימה
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JoVE 杂志 神经科学
The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry
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Assay Neuroblast: Assay לדור והעשרה של ובתאים העצבית הבחנה בין צאצאיהם גזע עצביים Cell שימוש cytometry זרימה

15,331 Views

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15:40 min

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April 22, 2012

DOI:

10.3791/3712-v

DOI:
10.3791/3712-v

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15:40 min
April 22, 2012

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2 Views
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1Department of Neurosurgery,The University of Florida, 2Laboratory for Stem Cell Research, Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran

Tags

Neuroblast AssayGeneration Of Neuronal Progenitor CellsDifferentiating Neural Stem CellsFlow CytometryNeurosphere AssayCentral Nervous SystemAstrocytesOligodendrocytesNeuronsIn Vitro StudiesDrug ScreeningNeurotoxicologyElectrophysiologyCell Replacement TherapyHeterogeneity Of NSC ProgenyLow Production Of Neurons And OligodendrocytesPredominance Of AstrocytesClinical ApplicationsPurification Of Immature NeuronsFluorescence Activated Cell Sorting (FACS)Murine NSC ProgenyE14 Mouse Ganglionic Eminences

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Azari, H., Sharififar, S., Fortin, J. M., Reynolds, B. A. The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry. J. Vis. Exp. (62), e3712, doi:10.3791/3712 (2012).

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