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Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
JoVE 杂志
生物学
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JoVE 杂志 生物学
Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer

DOI:
10.3791/3659-v

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10:58 min

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November 17, 2011

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1Department of Molecular and Cell Biology,Life Technologies , 2Life Technologies

Chapters

  • 00:05Title
  • 01:26Staining Cells with Tali Viability Kit – Dead Cell Red
  • 02:16Cell Viability Assay Using the Tali Image-Based Cytometer
  • 05:32Setting Thresholds and Gates
  • 09:33Tali Image-Based Cytometry vs. Flow Cytometry
  • 10:12Conclusion

Summary

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This protocol describes how to perform cell viability and fluorescence expression assays using the Tali Image-Based Cytometer.

Tags

GFP ExpressionViability AssessmentTali Image-based CytometerFlow CytometryFluorescence MicroscopyQuantitative MeasurementsVisualizationImage-based CytometryCell Viability AssayGFP Expression AssayTali InstrumentBenchtop Assay PlatformSuspension Cell-based AssaysApoptosis AnalysisPropidium Iodide

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