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Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
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JoVE 杂志 生物工程
Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

DOI:
10.3791/3641-v

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07:04 min

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March 02, 2012

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,
1Department of Biology,University of Victoria , 2Department of Mechanical Engineering, Division of Medical Sciences,University of Victoria

Chapters

  • 00:05Title
  • 01:31Day One: Making Fibrinogen Solutions and Overnight Dialysis
  • 02:57Day 2: Polymerization of Fibrin Scaffolds and Seeding of Stem Cells into Scaffolds
  • 06:01Representative Fibrin Scaffold Results
  • 06:38Conclusion

Summary

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This work details the preparation of 3D fibrin scaffolds for culturing and differentiating plutipotent stem cells. Such scaffolds can be used to screen the effects of various biological compounds on stem cell behavior as well as modified to contain drug delivery systems.

Tags

3D Fibrin ScaffoldsStem Cell Culture ApplicationsStem Cells3D MicroenvironmentsStem Cell NicheBiomaterial ScaffoldsPolystyreneCell PolarityNaturally Derived BiomaterialsSynthetic BiomaterialsMechanical PropertiesChemical PropertiesReproducibilityNatural BiomaterialsExtracellular MatrixCell AdhesionFibrin Scaffolds

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