Journal
/
神经科学
/
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures

DOI:
10.3791/3637-v

•

07:59 min

•

March 21, 2012

•

,
1Anatomical Institute, Department of Biomedicine,University of Basel

Chapters

  • 00:05Title
  • 01:48Setting up Organotypic Cerebellar Slice Cultures
  • 03:02Fixation and Immunostaining of Organotypic Cerebellar Slice Cultures
  • 04:52Measurement of Purkinje Cell Dendritic Tree Size and Branch Points
  • 06:07Representative Results for the Purkinje Cell Dendritic Tree Size and Branching
  • 07:23Conclusion

Summary

自动翻译

自动翻译

We present a protocol that permits to view and to quantitatively asses the morphology of the dendritic tree of individual Purkinje cells grown in organotypic cerebellar slice cultures. This protocol is intended to promote studies on the mechanisms of Purkinje cell dendritic development.

Tags

Purkinje CellDendritic MorphologyOrganotypic Slice CulturesSagittal PlanePostnatal PeriodAnti-Calbindin D28KEGFPJackson LabsCerebellar Slice CulturesDendritic DevelopmentDendritic ExpansionQuantitative EvaluationDendritic Tree SizeBranch Point NumbersAnti-calbindin StainedProtein Kinase C (PKC) Acti

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/3637?article_type=v

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury
check_url/cn/3637?article_type=v

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
check_url/cn/3637?article_type=v

Organotypic Hippocampal Slice Cultures
check_url/cn/3637?article_type=v

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
check_url/cn/3637?article_type=v

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
check_url/cn/3637?article_type=v

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
check_url/cn/3637?article_type=v

Organotypiske Slice Kulturer af Embryonale ventrale midthjernen: et system til undersøgelse Dopaminerge Neuronal Udvikling In vitro</em
check_url/cn/3637?article_type=v

Post-embedding immunogold mærkning af synaptiske proteiner i hippokampalskivekulturer
check_url/cn/3637?article_type=v

Organotypiske skivekulturer at studere oligodendrocyt Dynamics og myelinering
check_url/cn/3637?article_type=v

Analysen af ​​neurovaskulære Remodeling i Entorhino-hippocampus organotypisk skivekulturer

Read Article