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Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster
Chapters
- 00:05Title
- 01:30Constructing the Fly Brain Live Imaging and Electrophysiology Stage or “FLIES”
- 02:00Preparing the Fly for Observation
- 04:41Stimulating the Proboscis
- 05:28Simultaneous Recording of the Proboscis Extension Reflex and Calcium Imaging
- 06:22Conclusion
The fruit fly, Drosophila melanogaster, extends its proboscis for feeding, responding to a sugar stimulus from its proboscis or tarsus. I have combined observations of the proboscis extension response (PER) with a calcium imaging technique, allowing us to monitor the activity of neurons in the brain, simultaneously with behavioral observation.
Tags
Simultaneous RecordingCalcium SignalsIdentified NeuronsFeeding BehaviorDrosophila MelanogasterNeuronal ActivityBehavior AnalysisGenetically Encoded Calcium IndicatorsGCaMP1Optogenetic TechniquesThermogenetic TechniquesActivating Identified NeuronsCritical Neurons For Feeding BehaviorProboscis Extension ResponsePERSweet StimulusSensory CellsSuboesophageal GanglionSOGBehavioral ObservationExperimental System
