Journal
/
神经科学
/
Storstilet Registrering af neuroner ved Flytbare Silicon Probes at opføre sig Gnavere
JoVE 杂志
神经科学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Storstilet Registrering af neuroner ved Flytbare Silicon Probes at opføre sig Gnavere

DOI:
10.3791/3568-v

•

17:37 min

•

March 04, 2012

•

, , , , , , , , , , , , , ,
1Center for Molecular and Behavioral Neuroscience,University of New Jersey, 2Center for Interdisciplinary Research in Biology,Collège de France, 3Janelia Farm Research Campus,Howards Hughes Medical Institute, 4Deptartment of Psychology,University of Wisconsin at Milwaukee

Chapters

  • 00:02Title
  • 01:13Construction of the microdrive
  • 04:25Preparing the silicon probe
  • 05:55Affixing the probe to the microdrive
  • 06:37Preparing the skull
  • 10:53Preparing the brain surface
  • 12:40Implanting the probe
  • 14:07Building the on-head Faraday cage
  • 15:49Recording from the freely moving animal
  • 17:00Conclusion

Summary

自动翻译

自动翻译

Vi beskriver metoder til storstilet registrering af flere enkelte enheder og lokale felt potentiale i at opføre gnavere med silicium sonder. Drive fabrikation, probe tilknytning til drevet og sonde implanterings processer er illustreret i tilstrækkelige oplysninger til let replikation.

Tags

神经科学Large-scale RecordingMovable Silicon ProbesBehaving RodentsNeural AssembliesInput-output RelationshipsSpatial SelectivityTemporal ResolutionMechanistic AnalysisNeural EnsemblesMultiple-site RecordingSilicon-based DevicesMicro-machined Silicon ProbesCustom-made Accessory ComponentsPreamplifiersPrinted Circuit BoardsFlexible CablesBonding ServiceImplantation4-shank Probe32-site ProbePolyimide CableMovable Microdrive

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/3568?article_type=v

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat
check_url/cn/3568?article_type=v

Retrograd fluorescensmærkning Tillader Målrettet Extracellular Single-unit Optagelse fra Identificerede Neuroner<em> In vivo</em
check_url/cn/3568?article_type=v

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
check_url/cn/3568?article_type=v

Optagelse Gamma Band Svingninger i Pedunculopontine Nucleus neuroner
check_url/cn/3568?article_type=v

Storstilet rekonstruktioner og Independent, Uvildige Clustering Baseret på Morfologiske Metrics at klassificere Neuroner i Selektive populationer
check_url/cn/3568?article_type=v

Kvantificering af endosom og lysosommotiliteter i dyrkede neuroner ved anvendelse af fluorescerende prober
check_url/cn/3568?article_type=v

Implantation af kronisk silicium sonder og optagelse af hippocampus sted celler i en beriget løbebånd apparater
check_url/cn/3568?article_type=v

Ratiometric Calcium billeddannelse af enkelte neuroner i opfører sig Caenorhabditis Elegans
check_url/cn/3568?article_type=v

Konstruktion af en forbedret multi triode Hyperdrive for storstilet neurale optagelse i opfører sig rotter
check_url/cn/3568?article_type=v

Præparater og protokoller for hele cellen Patch klemme optagelsen af Xenopus laevis Tectal neuroner

Read Article