Journal
/
生物工程
/
Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
JoVE 杂志
生物工程
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物工程
Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

DOI:
10.3791/3520-v

•

10:08 min

•

July 25, 2012

•

, ,
1Department of Chemical Engineering,Stanford University

Chapters

  • 00:05Title
  • 01:40Flow Cell Preparation
  • 02:52Magnetic Tweezers Set-up and Calibration
  • 05:55Collagen Peptide Attachment to Flow Cells
  • 07:00Force Proteolysis Assay
  • 07:56Results: The Effect of Force on Enzymatic Proteolysis
  • 08:46Conclusion

Summary

自动翻译

自动翻译

In this article we describe the use of magnetic tweezers to study the effect of force on enzymatic proteolysis at the single molecule level in a highly parallelizable manner.

Tags

MultiplexedSingle-moleculeForceProteolysisMeasurementsMagnetic TweezersGenerationDetectionMechanical ForcesCell PhysiologyCancer MetastasisAtherogenesisWound HealingExtracellular Matrix (ECM)Enzymatically RemodelForces On ECMBiological ImportanceMedical ImportanceSingle Molecule TechniquesOptical TrappingAtomic Force MicroscopyMagnetic Tweezers (MT)Low CostHigh ThroughputPN (piconewton)Millisecond Temporal ResolutionEnzyme MechanismHighly Parallelizable AssayTrimeric Collagen PeptideMatrix Metalloproteinase 1 (MMP-1)Substrates And Proteases

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/3520?article_type=v

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
check_url/cn/3520?article_type=v

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays
check_url/cn/3520?article_type=v

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
check_url/cn/3520?article_type=v

Magnetic Tweezers for the Measurement of Twist and Torque
check_url/cn/3520?article_type=v

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
check_url/cn/3520?article_type=v

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
check_url/cn/3520?article_type=v

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
check_url/cn/3520?article_type=v

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
check_url/cn/3520?article_type=v

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
check_url/cn/3520?article_type=v

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Read Article