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MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson’s Disease
JoVE 杂志
医学
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JoVE 杂志 医学
MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson’s Disease

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson’s Disease

DOI:
10.3791/3445-v

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16:57 min

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February 14, 2012

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, ,
1Department of Pharmaceutical Biosciences,Uppsala University, 2Department of Chemical and Biological Engineering,Chalmers University of Technology

Chapters

  • 00:05Title
  • 02:02Tissue Preparation
  • 03:40Matrix Application
  • 05:24MALDI MS Data Acquisition, Processing, and Evaluation
  • 12:29Neuropeptide Validation
  • 14:18Representative MALDI-MS Results
  • 15:55Conclusion

Summary

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自动翻译

Dopamine replacement pharmacotherapy using L-DOPA is the most commonly used symptomatic treatment of Parkinson’s disease, but is accompanied by side effects including involuntary abnormal movements, termed dyskinesia 1. Here, a protocol for MALDI imaging mass spectrometry is presented that detects changes in rat brain neuropeptide levels related to dyskinesia.

Tags

MALDI Imaging Mass SpectrometryNeuropeptidesParkinson’s DiseaseIMSMolecular SpeciesMALDI MatrixIntact PeptidesProteinsMass AnalyzerMALDI TOF/TOF Mass SpectrometerImaging TechniquesMolecules Of InterestUnsupervised AnalysisMolecular SpecificitySensitivityRegion-specific Distribution ProfilesAnimal Model Parkinson’s DiseaseNeurodegenerative DiseaseDopamine ReplacementL-DOPA-induced Dyskinesias (LID)Opioid Precursor Prodynorphin MRNADynorphin PeptidesNeurotransmission

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