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Regissert Cellular Self-Assembly å dikte Cell-Avledet Tissue Rings for Biomekaniske Analysis and Tissue Engineering
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering

Regissert Cellular Self-Assembly å dikte Cell-Avledet Tissue Rings for Biomekaniske Analysis and Tissue Engineering

DOI:
10.3791/3366-v

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08:00 min

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November 25, 2011

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, , ,
1Biomedical Engineering Department,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 01:13Cell Seeding Mold Fabrication
  • 02:41Cell Culture and Ring Seeding
  • 03:35Tissue Ring Harvesting and Thickness Measurements
  • 04:02Mechanical Testing of Rings
  • 04:47Tissue Ring Assembly to Fabricate Tissue Tubes
  • 07:12Conclusion

Summary

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Denne artikkelen skisserer en allsidig metode for å skape celle-stammer vev ringer av cellulære selv-montering. Glatte muskelceller seeded inn ringformet agarose brønner samlet og kontrakt for å danne robuste tredimensjonale (3D) vev innen 7 dager. Millimeter-skala vev ringene er bidrar til mekanisk testing og fungere som byggesteiner for vev montering.

Tags

Directed Cellular Self-assemblyBiomechanical AnalysisCoronary Artery Bypass SurgeryAutologous Donor VesselsLiving Vascular ModelsCell-based MethodsSmooth Muscle CellsAgarose WellsMechanical AnalysisPhysiological AnalysisBiochemical AnalysisHistological AnalysisUltimate Tensile Strength

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