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Instrueret Cellular saml-selv at fabrikere Cell-afledt Tissue Ringe til Biomekanisk analyse og Tissue Engineering
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering

Instrueret Cellular saml-selv at fabrikere Cell-afledt Tissue Ringe til Biomekanisk analyse og Tissue Engineering

DOI:
10.3791/3366-v

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08:00 min

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November 25, 2011

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, , ,
1Biomedical Engineering Department,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 01:13Cell Seeding Mold Fabrication
  • 02:41Cell Culture and Ring Seeding
  • 03:35Tissue Ring Harvesting and Thickness Measurements
  • 04:02Mechanical Testing of Rings
  • 04:47Tissue Ring Assembly to Fabricate Tissue Tubes
  • 07:12Conclusion

Summary

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Denne artikel skitserer en alsidig metode til at oprette celle-deriverede væv ringe af cellulære selvsamling. Glatte muskelceller seedet til ringformet agarose brønde samlet og kontrakt for at danne robuste tre-dimensionelle (3D) væv inden for 7 dage. Millimeter-skala væv ringe er befordrende for mekanisk afprøvning og tjene som byggesten for væv samling.

Tags

Directed Cellular Self-assemblyBiomechanical AnalysisCoronary Artery Bypass SurgeryAutologous Donor VesselsLiving Vascular ModelsCell-based MethodsSmooth Muscle CellsAgarose WellsMechanical AnalysisPhysiological AnalysisBiochemical AnalysisHistological AnalysisUltimate Tensile Strength

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