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Organotypiske Slice Kulturer af Embryonale ventrale midthjernen: et system til undersøgelse Dopaminerge Neuronal Udvikling In vitro</em
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神经科学
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JoVE 杂志 神经科学
Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

Organotypiske Slice Kulturer af Embryonale ventrale midthjernen: et system til undersøgelse Dopaminerge Neuronal Udvikling In vitro</em

DOI:
10.3791/3350-v

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07:33 min

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January 31, 2012

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,
1Institute of Reconstructive Neurobiology,University of Bonn

Chapters

  • 00:05Title
  • 01:00Preparation for Dissection
  • 03:00Embryonic Brain Preparation and Vibratome Sectioning
  • 05:09Slice Culture and Time-lapse Imaging
  • 06:21Results: Dopaminergic Neuronal Development in Organotypic Slice Cultures of Embryonic Ventral Midbrain
  • 07:04Conclusion

Summary

自动翻译

自动翻译

En metode til at generere organotypiske skiver fra E12.5 murine embryonale midthjernen er beskrevet. Den organotypiske skive kulturer kan bruges til at observere opførslen af ​​dopaminerge neuroner eller andre ventral midthjernen neuroner.

Tags

Organotypic Slice CulturesEmbryonic Ventral MidbrainDopaminergic Neuronal DevelopmentIn VitroMouse Model OrganismBrain DevelopmentMolecular DataGenetic DataOrganotypic Slice Cultures Of Embryonic BrainsMurine Brain DevelopmentEx-vivo ManipulationTransgenic MiceGene ExpressionFluorescent ProteinsTime-lapse ImagingCerebral Cortex DevelopmentForebrainVentral Brain AreasNeuronal ClustersMigratory TrajectoriesFinal Position

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