This content is Free Access.
Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
15,960 Views
•
09:31 min
•
December 06, 2011
DOI:
Summary
Automatically generated
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This physiological approach allows observations of subpopulations and/or individual neurons in a living tissue and is convenient for receptor-ligand identification.
Explore More Videos
Pheromone SensingMousePheromonesIntra-species CommunicationBiological FluidsUrinePheromonal CommunicationKin InteractionsHierarchical OrganizationsSexual InteractionsSurvival Of SpeciesVomeronasal Organ (VNO)Nasal CavityCartilaginous CapsuleSensory NeuroepitheliumVomeronasal Bipolar Sensory Neurons (VSNs)DendriteMicrovilliChemoreceptorLigandsVomeronasal Receptor FamiliesV1RsV2Rs
Read Article
Cite this Article
Brechbühl, J., Luyet, G., Moine, F., Rodriguez, I., Broillet, M. Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation. J. Vis. Exp. (58), e3311, doi:10.3791/3311 (2011).
Copy