This content is Free Access.
MISSIE LentiPlex Gepoolde shRNA Library Screening in zoogdiercellen
18,369 Views
•
09:12 min
•
December 21, 2011
DOI:
Summary
Automatically generated
Hier gebruiken we een menselijke LentiPlex samengevoegde bibliotheek en traditionele sequencing methoden om gen-doelstellingen het bevorderen van overleving van de cel te identificeren. Laten we zien hoe op te zetten en deconvolute een LentiPlex scherm en valideren van de resultaten.
Explore More Videos
MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes
Read Article
Cite this Article
Coussens, M. J., Corman, C., Fischer, A. L., Sago, J., Swarthout, J. MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells. J. Vis. Exp. (58), e3305, doi:10.3791/3305 (2011).
Copy