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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Materials Science Program,University of Wisconsin-Madison, 3Department of Neurological Surgery,University of Wisconsin-Madison, 4Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine,University of Wisconsin-Madison
Chapters
- 00:05Title
- 01:57BTSC Neurosphere Dissociation
- 03:00Fabrication of Bi-layered su-8 Master
- 04:42Molding of the PDMS Stamp
- 05:31Assembly of Microfluidic Devices and Cell Culturing
- 07:04Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
- 07:46Time-lapse Recordings of Migrating BTSC
- 08:40Conclusion
Un dispositivo de microfluidos en compartimientos para la investigación de la migración de células madre del cáncer se describe. Esta novedosa plataforma crea un microambiente celular viable y permite la visualización microscópica de locomoción de células vivas. Muy móviles células cancerosas se limitan a estudiar los mecanismos moleculares de la infiltración agresivo, que puede conducir a terapias más eficaces en el futuro.
Tags
Cancer Stem Cell MigrationCompartmentalizing Microfluidic DevicesLive Cell Imaging癌症研究Cellular MigrationAggressive Cancer Cell InvasionMetastasisTherapeutic ResistanceGlioblastoma Multiforme (GBM)Primary Malignant Adult Brain TumorGBM InfiltrationTumor MicroenvironmentCancer Cell Migration MechanismsBrain Tumor Stem Cell (BTSC)Therapeutic ResistanceRecurrenceCell Migratory MechanismsConventional Migration AssaysMicrofluidic DevicesSingle Cell Analysis
