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FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

DOI:
10.3791/3289-v

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09:38 min

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August 05, 2011

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, , ,
1Biotechnology Research Institute,AUT University and KODE Biotech Ltd, 2Shemyakin Institute of Bioorganic Chemistry RAS,Moscow, Russia

Chapters

  • 00:05Title
  • 01:06Preparation of FSL Constructs
  • 01:49Insertion of FSL Construct(s) into Membranes
  • 02:39FSL-labeling Can Be Applied to a Diverse Variety of Cell Types
  • 09:24Conclusion

Summary

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Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.

Tags

FSL ConstructsBiological MarkersModify Cell SurfacesModify Virion SurfacesViabilityGenetic EngineeringCovalent AttachmentChemical MoietiesSurface ModificationHarmlessly Modify Cell SurfacesKODE TechnologyFunctional Head GroupSpacerLipid TailDispersible In WaterMembrane IncorporationSaccharidesBlood Group-related DeterminantsSialic AcidsHyaluronan PolysaccharidesFluorophoresBiotinRadiolabelsPeptides

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