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人类神经祖细胞和神经元小分子诱导人类胚胎干细胞的多能干高效的推导

JoVE 杂志
神经科学

This content is Free Access.

JoVE 杂志神经科学

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

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人类神经祖细胞和神经元小分子诱导人类胚胎干细胞的多能干高效的推导

DOI:

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10:47 min

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October 28, 2011

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Xuejun H. Parsons², Yang D. Teng⁴, James F. Parsons², Evan Y. Snyder^2,5, David B. Smotrich^2,6, Dennis A. Moore²

¹San Diego Regenerative Medicine Institute, ²Xcelthera, ³Department of Neurosurgery,Harvard Medical School, ⁴Division of SCI Research, VA Boston Healthcare System, ⁵Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, ⁶La Jolla IVF

Chapters

00:05Title
01:27Media Preparation and Plate Coating
02:33Passaging and Seeding Undifferentiated Human ES Cells
04:31Neural Induction in Adhesive Culture with Retinoic Acid
05:42Differentiation in Suspension Culture and Maturation to Neuronal Phenotype after Attachment
08:08Direct Neuronal Differentiation of Feeder- and Serum- Free Pluripotent Human ES Cells WIth Retinoic Acid Induction
10:11Conclusion

Summary

自动翻译

我们已经建立了诱导多能干人类胚胎干细胞与小分子物质，使推导的大量供应人类神经祖细胞和神经细胞类型，在发展中国家中枢神经系统神经修复定义的条件下保持直接的神经母细胞的协议。

Tags

Human Neuronal Progenitors Neurons Pluripotent Human Embryonic Stem Cells Small Molecule Induction Cell-based Therapies Central Nervous System (CNS)Neural Stem Cells Expansion Ability Plasticity Trophic And Neuroprotective Molecules Pluripotent HESCs Neurological Disorders Differentiation Potential Developmental Study Clinical Translation Multi-lineage Inclination Germ Layer

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