需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
Chapters
- 00:05Title
- 00:08Introduction
- 01:35Instrumentation
- 03:37Dissociation of Olfactory Sensory Neurons
- 07:51Recording
- 09:37Representative Results
- 11:09Conclusion
Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.
Tags
Flash PhotolysisCaged CompoundsOlfactory Sensory NeuronsPatch-clamp RecordingsPhotolysis Of Caged CompoundsOlfactory TransductionDissociated Mouse Olfactory Sensory NeuronsCiliaOdorant BindingReceptorsCAMPCyclic Nucleotide-gated (CNG) ChannelsCa EntryCa-activated Cl ChannelsMouse Olfactory EpitheliumFlash LampUltraviolet FlashesWhole-cell Voltage-clamp Configuration
