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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

DOI:
10.3791/3155-v

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06:00 min

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November 18, 2011

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, , , ,
1RIKEN BioResource Center

Chapters

  • 00:05Title
  • 01:13Vitrification of 2-cell Mouse Embryos
  • 02:22Thawing Vitrified 2-cell Mouse Embryos
  • 05:03Representative Results
  • 05:30Conclusion

Summary

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An ethylene glycol-based vitrification method for mouse embryos is described. It is advantageous to other methods in its simplicity and low embryonic toxicity, and therefore can be broadly applicable to many strains of mice, including inbred and gene-modified mice.

Tags

CryopreservationMouse EmbryosEthylene Glycol-based VitrificationBiomedical SciencesGenetic ModificationsSlow Freezing MethodsVitrification MethodsCryoprotectantsToxicityEmbryo DevelopmentRIKEN BioResource CenterMouse StrainsGenetic BackgroundsPhenotypesVitrification TechniqueEmbryo SurvivalCryopreservation SolutionEthylene GlycolDMSOFicollSucrose

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