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총 RNA의 분리 및 다운 스트림 차세대 시퀀싱 및 유전자 발현 프로 파일링에 대한 개별 마우스 뇌 영역의 Microdissection 아닌 레이저 캡처 현미경 접근법
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10:06 min
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November 13, 2011
DOI:
Summary
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RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.
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Non-laser Capture MicroscopyMicrodissectionMouse Brain RegionsTotal RNA IsolationNext-generation SequencingGene Expression ProfilingTechnological PlatformsNext-generation SequencingMicroarrayQRT-PCRHigh-resolution RNA SequencingRNA-SeqTranscript LevelsAlternative SplicingMicro RNAsWhole Transcriptome ProfilingPhenotypically Distinct GroupsMolecular MechanismsPhysiological StatesDisease ProgressionNext-generation Sequencing IntegrationGene Expression StudiesMouse ModelsBrain Nuclei PrecisionLaser Capture Microscopy (LCM)Sample Excision
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Atkins, N., Miller, C. M., Owens, J. R., Turek, F. W. Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling. J. Vis. Exp. (57), e3125, doi:10.3791/3125 (2011).
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