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Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

DOI:
10.3791/3111-v

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09:42 min

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November 07, 2011

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,
1Disease Mechanism Research Core,RIKEN Brain Science Institute, 2Graduate School of Science and Engineering,Saitama University

Chapters

  • 00:05Title
  • 01:13Generating Mosaics by Heat Shock
  • 02:30Screening and Imaging of Dendrite Clones
  • 03:50Larval Dissection and Tissue Preparation
  • 06:14Mounting Larval Fillets
  • 08:03Confocal Images of in vivo and Fixed Preparations
  • 08:51Conclusion

Summary

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自动翻译

The dendritic arborization sensory neurons of the Drosophila larval peripheral nervous system are useful models to elucidate both general and neuron class-specific mechanisms of neuron differentiation. We present a practical guide to generate and analyze dendritic arborization neuron genetic mosaics.

Tags

Morphological AnalysisDrosophila LarvalPeripheral Sensory NeuronDendritesAxonsGenetic MosaicsNervous System DevelopmentNeuron PositionNeuron IdentityDendritic DevelopmentAxonal WiringDendritic Arborization (DA)Sensory NeuronsPeripheral Nervous System (PNS)Genetic ModelsNeuron DifferentiationClass-specific MechanismsDendritic MorphologyMolecular MechanismsFruit FlyLarval CuticleHigh Resolution VisualizationComparative AnalysisMorphometric Statistical AnalysisDA Neuron ActivityLocomotion Central Pattern Gene

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