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Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

DOI:
10.3791/3024-v

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09:30 min

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August 15, 2011

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, , , ,
1Lab for Molecular Mechanisms of Thalamus Development,RIKEN Brain Science Institute

Chapters

  • 00:05Title
  • 02:08Capillary and Micro-Electrode Preparation
  • 03:48Plasmid DNA Preparation
  • 04:47Surgery, Injection and Electroporation
  • 07:03In utero Cortical and Deep-tissue Plasmid Electroporation
  • 08:34Conclusion

Summary

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A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.

Tags

MouseIn Utero ElectroporationGene TransfectionDeveloping BrainGene TransferKnock-outKnock-inSpecific CRE LineTransfection MethodCandidate GenesTransgenic AnimalsBrain AreasEmbryonic LethalityElectrodesOptic Fiber Optic Light CableDNA Solution InjectionVentriclesNeedle Type ElectrodesBrain Area Patterning

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