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Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
JoVE 杂志
生物工程
Author Produced
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JoVE 杂志 生物工程
Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

DOI:
10.3791/2909-v

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10:00 min

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June 05, 2011

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1Department of Molecular and Cellular Biology,University of California Davis

Chapters

  • 00:02Title
  • 00:05Introduction
  • 00:37Materials
  • 01:25Fabrication
  • 05:13Sample Preparation
  • 06:18Using the Cryo-Light Stage
  • 07:51Stage Stability
  • 08:35Results
  • 09:13Conclusion

Summary

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We demonstrate the fabrication of a low-cost cryogenic stage designed to fit most reflected light microscopes. This lab-built cryogenic stage enables efficient and reliable correlative imaging between cryo-light and cryo-electron microscopy.

Tags

Cryo-light MicroscopyCryo-electron MicroscopyCorrelated MicroscopyCellular DynamicsUltrastructureCryogenic StageCryo-LMCryo-EMSample PreservationVitrification ProcessCorrelative ImagingInexpensive Cryogenic StageReflected Light MicroscopesLong Working Distance Air ObjectivesCarbon Coated Cryo-EM GridsMulti-technique Imaging

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