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禽流感监测与FTA卡:实地方法,生物安全,和运输问题解决了
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
DOI:

12:09 min

August 02, 2011

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Chapters

  • 00:05Title
  • 02:17Duck Trapping and Cloacal Swabbing
  • 04:02Viral RNA Isolation and RT-PCR
  • 07:28Screening for AI-positive Samples and Purification of Targeted Fragments from Gel
  • 08:40Screening and Identification of PCR Products
  • 09:21Representative Results
  • 10:41Conclusion

Summary

自动翻译

维护,检测和序列禽流感病毒的RNA的方法进行了验证和扩展,从鸟类的自然粪便样本。这种技术消除了保持冷静的链和处理传染性病毒的必要性和可应用在一个96孔高通量设置。

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