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Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
JoVE 杂志
生物学
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JoVE 杂志 生物学
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

DOI:
10.3791/2777-v

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10:27 min

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July 30, 2011

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, ,
1Department of Biological Sciences,Southwestern Oklahoma State University

Chapters

  • 00:05Title
  • 01:12RNA Preparation
  • 03:32Microarray Hybridization
  • 05:32Microarray Analysis
  • 08:44Finding Gene Expression Changes in the vsm-1 Synaptogenesis Mutant
  • 10:04Conclusion

Summary

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Microarray analysis was conducted to determine genetic expression profiles in C. elegans, and real-time PCR was used to validate and quantify microarray data.

Tags

Genetic Expression ProfilesC. ElegansMicroarrayReal-time PCRSynapsesPresynaptic Active ZonePostsynaptic TerminalNeurotransmittersReceptorsVSM-1Exocytosis RegulationSynaptic ConnectivitySynaptogenesisDouble-labeled Microarray AnalysisGene Expression ProfilesRNA IsolationCDNA Reverse TranscriptionDNA MicroarraysFluorescent Probe HybridizationMajor Sperm Protein Family (MSP)Nematode Oocyte MaturationOvulationPresynaptic Bouton Number And Size

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